ABSTRACT
Topiramate is an anticonvulsant used to treat seizures and prevent migraines. The aim of this study was to develop andvalidate a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantifyTopiramate in its formulation. Acetonitrile and ammonium acetate were used as a mobile phase (85:15 v/v ratio),and isocratic elution mode was used for separation using Zorbax RP-C18Column (50 mm × 4.6 mm i.d., 5 µ) asa stationary phase. The standard calibration curve ranges from 1 to 1,000 ng/ml with a correlation coefficient of0.9990 (R2). The detection and quantification limits were obtained at 0.5 and 1.0 ng/ml, respectively. The total runtimeof chromatographic separation was found to be 2.0 minutes with a retention time of 1.23 minutes. The percentagerecovery studies were found to be 90.3%–99.3%. The developed method was found to be simple and sensitive and canbe used for the estimation of Topiramate in bulk and its pharmaceutical formulations.
ABSTRACT
The current study reports on a mass spectrometric method for the quantification of gemfibrozil drug substance in itsformulation. Isocratic method was used for separation with help of Zorbax SB C18 column (4.6 × 50 mm, 3 µm) using atriple quadrupole mass detector in multiple reaction monitoring (MRM) mode and electrospray ionization as ionizationsource. The resulting runtime was found to be 3 minutes and the analyte elutes at 1.201 minutes 10-mM ammoniumformate was adjusted to pH 3.5: methanol (20:80 V/V) was used as mobile phase. Increased sensitivity from selectiveion monitoring (SIM) to MRM resulted in decreased quantitation limit (LOQ) and 0.5 ng/ml as detection limit (LOD).The method was validated in a range of 1–50 ng/ml and a correlation coefficient of 0.999. The method recoverywas found to be in the range of 94.03 % to 100.02 %. The developed method was validated as per the InternationalConference on Harmonisation (ICH) guidelines
ABSTRACT
Background: Obesity is a medical condition in which excess body fat has accumulated to the extent that it may have a negative effect on health.1 People are generally considered obese when their body mass index (BMI), a measurement obtained by dividing a person's weight by the square of the person's height, is over 30 kg/m2, with the range 25–30 kg/m2 defined as overweight.Aims and objectives were to estimate the prevalence of overweight and obesity among the study population and to compare the study results between urban and rural area population.Methods: A community based cross-sectional study from April 2008 to May 2009 (1 year) conducted at urban health centre, Harazpenta and rural health centre, Patancheruvu. Total of 1409 persons in the age group of above 15 years were involved in this study. Data collection by using pre-designed, pretested questionnaire. Data analysis by using MS office 2016. Epi info2016, rates, ratios, proportions and Chi-square tests were used.Results: Among urban population prevalence of obesity was 12.7% compared to 5.4% in rural area. The prevalence of pre obesity was more among males (26.2%) in urban area than rural area (16.72%). In the study population 42.9% of the individual who had BMI>25 were from upper middle class in urban area, when compared to 43.3% with BMI>25 belongs to upper class in rural area.Conclusions: All over weight and obese subjects should be educated to know about the occurrence of various health problems due to overweight.